Names: Haloferax volcanii DS2: Accession numbers: NC_013964, NC_013965, NC_013966, NC_013967, NC_013968: Background: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / IFO 14742 / NCIMB 2012 / DS2) is a moderate halophilic archaeon isolated from … CetZ2 was also implicated in H. volcanii cell shape control. This practice is similar to that of other Archaea and, indeed, that of bacteria. (2020) Lynch et al. Researcher: molecular microbiologist. 1. Haloferax volcanii - Description and significance Microbiologist Benjamin Elazari Volcani first discovered Haloferax volcanii, a self-named extremophile, in the 1930s. It has a disc or cupped shape and reproduces by building cytoplasmic bridges with others in the Haloferax genus and sharing genetic information .As with most halophiles, it requires a high salt concentration in order to thrive. Our findings expandthe known roles of the FtsZ/tubulin superfamily to include archaeal cell shape dynamics, suggesting that a al role might predate cytoskelet eukaryotic cell evolution, and they support the premise that a major function of microbial rod-shape is to facilitate swimming. Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) Status. How ribosomes shape the proteome. It is easily grown in the lab. Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation Michiyah Collins 1, Simisola Afolayan 1, Aime B. Igiraneza 1, Heather Schiller 1, Elise Krespan 2, Daniel P. Beiting 2, Mike Dyall-Smith 3,4, Friedhelm Pfeiffer 4 and Mechthild Pohlschroder 1,* The paper, 'Cell division in the archaeon Haloferax volcanii relies on two FtsZ proteins with distinct functions in division ring assembly and constriction' by Iain G. Duggin, Yan Liao, Solenne Ithurbide and Christian Evenhuis (iThree Institute, UTS) and Jan Löwe (MRC Laboratory of Molecular Biology), has been published in Nature Microbiology. NaCl alone could not stabilize the isolated envelopes and divalent cations were absolutely required at concentrations which depended on that of NaCl and on the temperature. shapes, such as squares. Mass spectrometry revealed the pentasaccharide to comprise two hexoses, two hexuronic acids and an additional 190 Da saccharide. 1 Publication 2007; Hawkins, Malla, et al. salinarum, Haloferax sp., Halorubrum sp.) Despite sharing >60% sequence identity and the ability of VNG1048G to functionally replace AglM in vivo, these proteins behaved differently as salinity changed. Several haloarchaea are reported to be pleomorphic, while others exhibit remarkable shapes, such as squares. Recently, a novel membrane anchoring mechanism involving a peptidase, archaeosortase A (ArtA), and C-terminal lipid attachment of surface proteins was identified in the model archaeon Haloferax volcanii. Haloferax volcanii WR11 contains a plasmid of 3 kb (pHV11),representing aperfect toolwithwhichto studyhow environmental factors act on DNAtopology. (a) Representative phase-contrast image of wild type cells at a magnification of 1000x. Haloferax volcanii strains were grown at 45 °C on complete (Hv-YPC) or casamino acids (Hv-Ca) agar, or in Hv-YPC broth, as described previously (Allers et al. In routine liquid cultures of H. volcanii , both pleomorphic discoid (plate) and rod cell morphologies may be observed, occasionally with more unusual shapes [ 2–4 ]. This archaea is a halophile: it grows in environments with a very high salt concentration. Planktonic H. volcanii DS2 cells grown in shaking culture (Figure 1A) readily formed biofilms in typical rich media types Hv-YPC and Hv-Ca within several experimental systems that provided a solid plastic or glass substratum. Proteins recognized and processed byH. Haloferax volcanii Haloferax mediterranei Haloferax Halobacteriales Halobacteriaceae Archaea Haloarcula Halobacterium salinarum Halobacterium Dipterocarpaceae Halorubrum Bacillus Planococcus Bacteria Luffa. Deletion of one of these genes (cetZ1) in the haloarchaeon Haloferax volcanii abolished motility. Hfx volcanii) has been reported to display different cell shapes, including rod-shaped and plate-shaped cells [12,15,16]. These molecules are visualized, downloaded, and analyzed by users who range from … ... Involved in cell shape control. and two bacterial (Salicola sp., Halomonas sp.) Here, we have developed a reliable protocol for the study of the archaeon Haloferax volcanii. FtsZ1 and FtsZ2 colocalize to form the dynamic division ring. Haloferax volcanii, a halophilic archaeon, is a tractable model to study prokaryotic genome plasticity and the evolution of new chromosomes (Mullakhanbhai and Larsen 1975; Charlebois, et al. Haloferax volcanii (strain ATCC 29605 / DSM 3757 / IFO 14742 / NCIMB 2012 / DS2) is a moderate halophilic archaeon isolated from bottom sediment from the Dead Sea. It is named in honour of Benjamin Volcani who pioneered the microbiology of the Dead Sea. S-layer proteins and flagellins have been used successfully to study a variety of these modifications, including N-linked glycosylation, signal peptide removal and lipid modification. Recently, he identified and defined conditions for cell differentiation in Haloferax volcanii, and developed cell biology tools for this organism, combining them with well-established molecular genetic methods to develop a model for cell biology studies. We have generated an autofluorescence-free H. volcanii strain, evaluated several fluorescent proteins for their suitability to serve as single-molecule fluorescence markers and codon-optimized them to work under optimal H. volcanii cultivation conditions. Among the most studied haloarchaea, special attention has been given to Haloferax volcanii, a moderate halophile frequently used as a model organism for this domain [ 11, 12 ]. First isolated from deep sediments of the Dead Sea [ 13 ], optimal growth occurs at 1.7–2.5 M NaCl, 45°C, and at slightly acidic pH values [ 14 ]. Despite the harsh environmental conditions they endure, these extremophiles represent a great deal of the Earth’s biodiversity. Names: Haloferax volcanii DS2: Accession numbers: NC_013964, NC_013965, NC_013966, NC_013967, NC_013968: Background: Haloferax volcanii (strain ATCC 29605 / DSM 3757 / IFO 14742 / NCIMB 2012 / DS2) is a moderate halophilic archaeon isolated from … Haloferax volcanii (strain ATCC 29605 / DSM 3757 / IFO 14742 / NCIMB 2012 / DS2) is a moderate halophilic archaeon isolated from bottom sediment from the Dead Sea. Hfx. 2010). An extensive repertoire of genetic, molecular biological, and biochemical tools has been developed for this fast-growing, easily cultivated haloarchaeon, including expression vectors and gene-deletion strategies, including CRISPR. UofT Libraries is getting a new library services platform in January 2021. The RCSB PDB also provides a variety of tools and resources. salinarum and Hfx. Haloferax volcanii is a halophile originally discovered in the Dead Sea. Isolated from the Dead Sea in 1975, Haloferax volcanii thrives in high salt environments and has emerged as an important archaeal model system. Organisms adapted to life in extreme habitats (extremophiles) can further our understanding of the mechanisms of genetic stability, particularly replication and repair. … The model haloarchaeon, Haloferax volcanii, when first isolated, was described as mainly disc-shaped cells, with cell shape and size varying significantly . These molecules are visualized, downloaded, and analyzed by users who range from … Haloferax volcanii cells develop into structured colony biofilms and static liquid biofilms. It is unknown if this growth phase dependent cell shape alteration is a specific feature of Hfx. gen. masc. Haloferax volcanii (formerly Halobacterium volcanii) was first identified in the 1930s by microbiologist Benjamin Elazari Volcani, for whom the species is named . ... CetZ2 was also implicated in H. volcanii cell shape control. 1 Publication 5C and D); at all time points tested, We sequenced the ftsZ gene region of the halophilic archaeon Haloferax mediterranei and mapped the transcription start sites for the ftsZ gene. The archaeon Haloferax volcanii has a single flexible S-layer, two FtsZ homologues, and six CetZs. Organisms adapted to life in extreme habitats (extremophiles) can further our understanding of the mechanisms of genetic stability, particularly replication and repair. 2014 Jun;16(6):1779-92. doi: 10.1111/1462-2920.12385. The requirement of p-gvpC, p-gvpN, and p-gvpO gene expression for gas vesicle synthesis was assessed by transformation experiments using the VAC- species Haloferax volcanii as the recipient.
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